Production and Optimization of Exopolysaccharide from Thermophilic Bacteria

Exopolysaccharides (EPS) are the large molecular weight carbohydrate polymers extracted from higher plants, algae, fungi and bacteria. The thermophilic Bacillus zhangzhounesis 2CA licheniformis 2CS used in present study were isolated Çermik hot springs. growth conditions of strains designated as different basal media (M1, M2 M3), carbon sources concentrations yeast extract (%w v-1: 0.05, 0.1, 0.15 0.2) amount EPS produced investigated. In addition, phenol-sulfuric acid method Lowry to determine carbohydrates proteins within by bacteria, respectively. highest total dry for B. 2ÇS was obtained 121 mg M3 medium (0.2% + 1% sucrose), content 333.28 µg mL-1 protein 0.19 mL-1. When these two bacteria compared terms produced, found (1087.03 mL-1). antibacterial effects investigated against pathogenic microorganisms (E. coli, S. aureus, K. pneumoniae P. aeruginosa). It determined that activity E. coli (with 16 mm zone diameter) with sucrose).